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Aim To explore whether the polypeptide vaccines CKL9 and YL20 can induce immune response and anti-tumor effect on HER-2 (+) tumors in vitro and in vivo, and to provide suggestions for clinical use.Methods The proliferation of specific lymphocytes and cytotoxic T lymphocyte activity(CTL) stimulated by CKL9 and YL20 were studied with CCK-8 assay and LDH assay, and the antitumor activity of CKL9 and YL20 was evaluated in vivo.Results The lymphocyte proliferation was promoted by incubation with CKL9 and YL20, and the relative increase of cells was 11.1% and 16.7% respectively at the concentration of 50 mg·L-1 of CKL9 and YL20.The LDH assay confirmed the CTL effect induced by CKL9 and YL20 on HER2-positive tumor cells, not on HER2-negtive tumor cells.With an effector-target ratio of 80 ∶1, the inhibition of tumor cell by cytotoxic T lymphocyte stimulated by CKL9 and YL20 could reach 89.8% and 84.3%, respectively.The HER2(+) tumor cell N87 transplanted in Babes mice was inhibited by pre-immune polypeptide CKL9 and YL20.Conclusion The HER2-specific polypeptide vaccines CKL9 and YL20 could induce persistent specific CD4 and CD8 T cell immune and inhibit the growth of HER2 positive tumor cells.
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Objective To study the effect of music therapy on psychological state of cancer patients during treatment by high intensity focused ultrasound,as well as its influence on vital signs.Methods 157cases treated by high intensity focused ultrasound were randomly divided into the observation group(78cases)and the control group(79 cases).The control group received routine care,the observation group received the intervention of individual music therapy on the basis of routine care.The treatment effect was compared between the two groups.Results After the music intervention the observation group's breathing,heart rate,systolic and diastolic blood pressure were significantly different compared with those of the control group,After the music intervention the observation group's SAS,SDS scores were lower than those of the control group.Conclusions Music therapy can ease the anxiety and depression of cancer patients treated by high intensity focused ultrasound,the treatment can effectively improve the psychological and physiological regulation,make patients more comfortable and improve the quality of life of patients too.
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Objective To study the clinical effect of Liushen mixture combined with hyperoxia solution to treat the pressure sores of advanced cancer patients. Methods 43 cases of advanced cancer patients with pressure sores were randomly divided into the experimental group (21 patients)and the control group (22 patients). All patients received conventional care after debridement, the experimental group was treated by 30 to 60 Liushen pills grinded into powder reconciled with 3～8 ml 2% lidocaine to cover the affected area as external fomentation by sterile gauze which was soaked by hyperoxia solution, fixed with cling film,regular replacement The control group was coated with silver sulfadiazine sterile gauze and regular replacement. The cure rate, efficiency rate, and healing time of pressure sores of the two groups were observed respectively. Results In the experimental group the cure rate and efficiency rate were respectively 76.19% and 90.48%, and the cure rate and efficiency rate of the control group were 45.45% and 63.64%, the difference was statistically significant. In the experimental group the phase Ⅲ pressure sores' healing time was ( 13.60± 4.92)days, the healing time of the control group was(20.76 ± 5.51 )days, the difference was statistically significant. Conclusions Liushen pills and lidocaine mixture combined with hyperoxia solution to treat the pressure sores of advanced cancer patients have significant effect and are worthy of clinical application.